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Título del libro: Horizons In Neuroscience Research
Título del capítulo: Characterization of cytoarchitecture of dendrites and fiber neurons using the Golgi-Cox method: An overview

Autores UNAM:
LEONARDO RODRIGUEZ SOSA; MARIA GABINA CALDERON ROSETE;
Autores externos:

Idioma:
Inglés
Año de publicación:
2015
Palabras clave:

Hippocampus; Hyperglycemia; Prefrontal cortex; Prenatal stress; Sholl analysis; Subthalamic nucleus


Resumen:

In this chapter, we describe the purported Golgi-Cox method of impregnation designed to stain certain neurons in rats. This method has been widely used to evaluate the effects of experimental manipulations on the morphology of neurons in the central nervous system (CNS) associated with physiological modifications. Moreover, here we show how this staining procedure for light microscopy adapted to a camera Lucida together with the Sholl analysis, provides clear information about morphology, distribution, location, and intrinsic connections of neurons in the CNS. We focused on the study of the morphology of neurons and the extensive network of dendrites, in different regions of the CNS, under following three experimental conditions: the morphological changes in the hippocampus and nucleus accumbens after prenatal stress; reduction in the number of dendritic spines in the hippocampus due to hyperglycemia and rearrangement of the dendritic morphology of neurons in the prefrontal cortex and hippocampus after subthalamic lesion. From these observations, we may conclude that the Golgi-Cox method is a simple, accessible and efficient procedure which also provides a good resolution of the morphology of the fibers, as well as the spines in the dendrites of the neurons in the CNS. © 2015 Nova Science Publishers, Inc.


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