ERROR:SQLSTATE[HY000]: General error: 20018 Invalid column name 'CodigoInstitucion'. [20018] (severity 16) [SELECT PublicObrasAutores.NumeroEmpleado, PublicObrasAutores.ApellidoPaterno, PublicObrasAutores.ApellidoMaterno, PublicObrasAutores.Nombre, PublicObrasAutores.NombreCompleto FROM PublicObrasAutores LEFT JOIN PublicObrasAutoresFil ON PublicObrasAutores.RefPublicacion = PublicObrasAutoresFil.RefPublicacion WHERE PublicObrasAutores.RefPublicacion = '324049' AND PublicObrasAutores.NumeroEmpleado IS NULL AND PublicObrasAutoresFil.CodigoInstitucion IS NOT NULL;]
®
ERROR:SQLSTATE[HY000]: General error: 20018 Invalid column name 'CodigoInstitucion'. [20018] (severity 16) [SELECT PublicObrasAutores.NumeroEmpleado, PublicObrasAutores.ApellidoPaterno, PublicObrasAutores.ApellidoMaterno, PublicObrasAutores.Nombre, PublicObrasAutores.NombreCompleto FROM PublicObrasAutores LEFT JOIN PublicObrasAutoresFil ON PublicObrasAutores.RefPublicacion = PublicObrasAutoresFil.RefPublicacion WHERE PublicObrasAutores.RefPublicacion = '324049' AND PublicObrasAutores.NumeroEmpleado IS NULL AND PublicObrasAutoresFil.CodigoInstitucion IS NULL;]
®
ERROR:SQLSTATE[HY000]: General error: 20018 Invalid column name 'Institucion'. [20018] (severity 16) [SELECT Institucion FROM PublicObrasAutoresFil WHERE RefPublicacion = '324049']
®
ERROR:SQLSTATE[HY000]: General error: 20018 Invalid column name 'Institucion'. [20018] (severity 16) [SELECT Institucion FROM PublicObrasAutoresFil WHERE RefPublicacion = 324049 AND InstitucionPropia = 'S'
UNION
SELECT Adscripciones.Entidad FROM PublicacionesObras
JOIN PublicObrasAutores ON PublicacionesObras.Identificador = PublicObrasAutores.RefPublicacion
JOIN Adscripciones ON PublicObrasAutores.NumeroEmpleado = Adscripciones.NumeroEmpleado
WHERE (PublicacionesObras.FechaPublicacion BETWEEN Adscripciones.FechaDesde AND Adscripciones.FechaHasta OR (PublicacionesObras.FechaPublicacion >= Adscripciones.FechaDesde AND Adscripciones.FechaHasta IS NULL)) AND PublicacionesObras.Identificador= 324049]
SIIA Público
SISTEMA INTEGRAL DE INFORMACIÓN ACADÉMICA - PÚBLICO
Título del libro: Laboratory Methods In Cell Biology: Imaging Título del capítulo: RNA Imaging: Tracking in Real-Time RNA Transport in Neurons Using Molecular Beacons and Confocal Microscopy
RNAs are present within eukaryotic cells and are involved in several biological processes. RNA transport within cell compartments is important for proper cell function. To understand in depth the cellular processes in which RNA is involved requires a method that reveals RNA localization in real time in a sub-cellular context in living cells. In this protocol we describe a method for imaging RNA in living cells and in particular in neuronal cultures based on cell microinjection of molecular beacons in conjunction with confocal microscopy. This methodology overcomes some of the main obstacles for imaging RNA in live cells since microinjection allows the delivery of the probe to a desired cellular compartment and MBs bind with high specificity to its target RNA without inhibiting its function. The proper design of the MBs is essential to obtain RNA-MB association at the temperature of the cell cytosol. MBs design with other purposes in mind (such as PCR experiments) have a design that fac
