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Título del libro: Partial Or Complete Vestibular Recovery Is Related To The Presence Of Mitdna Specific Polymorphisms In Aminoglycoside Antibiotic Treated Female Patients

Autores UNAM:
BEATRIZ AGUILAR MALDONADO; LAURA MARIA ONGAY LARIOS; GRACIELA MEZA Y RUIZ;
Autores externos:

Idioma:
Inglés
Año de publicación:
2016
Palabras clave:

Aminoglycoside susceptibility; Audiometry; Electronystagmography; Gentamicin; Mitochondrial DNA polymorphisms; Otoacustic emissions; Posturography; Streptomycin; Vestibular ablation


Resumen:

Scope: With the aim of finding out a correlation between vestibular function alterations and the presence of polymorphisms in mitDNA isolated from patients treated with aminoglycoside antibiotics a comparative study was performed in 5 female patients treated with gentamicin for a short period of time. Materials And Methods. Clinical studies were attained to determine patient vestibular or auditory function. Analysis of mitochondrial DNA (mitDNA) was performed in blood samples of each patient by means of Molecular Biological techniques and the obtained nucleotide sequences were compared with standard reference sequence (Cambridge rCRS) for human mitDNA. Results. Clinical studies demonstrated that three of the patients treated with gentamicin and one treated with streptomycin showed vestibular ablation. In none of the patients auditory function was compromised. After a period of vestibular rehabilitation, only one patient completely recovered from vestibular damage, the rest presented partial vestibular rehabilitation. When mitDNA sequences were analyzed, changes were found in the five patients, some of them localized in the hypervariable regions 2 and 3; patients with vestibular damage presented more numerous changes than the patient whose vestibular function was unaffected. In mitDNA of the patient with no recovery after rehabilitation, changes were observed mainly in the region in which transcription factors usually bind therefore affecting importantly mitochondrial function which resulted in a permanent damage of vestibular function. Conclusion. Results obtained suggest that possibly some of the modifications found in the sequence of mitochondrial DNA could be responsible for the vestibular alterations caused by aminoglycoside and probably of the permanent damage to vestibular function. © 2016 by Nova Science Publishers, Inc. All rights reserved.


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