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Título del libro: 21st International Conference On Miniaturized Systems For Chemistry And Life Sciences, Microtas 2017
Título del capítulo: A microfluidic device to study single-cell calcium dynamics under time-varying stimuli

Autores UNAM:
ARTURO HERNANDEZ CRUZ;
Autores externos:

Idioma:
Inglés
Año de publicación:
2020
Palabras clave:

Calcium; Concentration Gradient Generator; Single-Cell; Time-Varying Stimuli


Resumen:

We present an integrated microfluidic device to study single-cell calcium signaling dynamics. Our device produces oscillating chemical signals, from which we can control their frequency and amplitude. The microfluidic device consists of a linear concentration gradient generator (CGG) that feeds into nine independent Pchambers. Each Pchamber comprises 492 Pwells that trap individual cells. Intracellular calcium dynamics were measured on Human Embryonic Kidney cells 293 (HEK-293) to test the capability of the device. Our platform could be used to study any single-cell signaling dynamics that relay on a fluorescent signal for its analysis. © 17CBMS-0001.


Entidades citadas de la UNAM: