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Título del libro: Transactions - 7th World Biomaterials Congress
Título del capítulo: Osteoblast behavior on amorphous carbon films

Autores UNAM:
SANDRA ELIZABETH RODIL POSADA; HIGINIO ARZATE;

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Autores externos:

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Idioma:

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Año de publicación:
2004

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Palabras clave:

Biomedical engineering; Bone; Carbon; Cells; Magnetron sputtering; Scanning electron microscopy; X ray analysis; Amorphous carbon films; Biomedical implants; Osteoblast; Vacuum chambers; Amorphous films

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Resumen:

Amorphous carbon (a-C) films were deposited on stainless steel substrates (SS) using a dc magnetron sputtering system attached to a high vacuum chamber. In vitro studies were carried out on the coated samples using human osteoblast cells. Human alveolar bone-derived cells (HABDC) were grown on a-C and SS samples to evaluate cellular attachment and proliferation, these tests were determined by direct cellular counting using spectroscopic techniques and a well defined standard curve. The percentage of osteoblasts attached, measured at 24 hours, attained maximum values for a-C films. Similarly, cellular proliferation evaluated at 3, 5 and 7 days showed and outstanding increase of osteoblast cells for a-C in contrast with uncoated steel. Cytotoxicity was evaluated by the MTT test after incubation periods of 3, 5 and 7 days. The absorbance values obtained for a-C resulted significantly higher than the positive control, indicating that the surface did not induce any toxic effect in short and longer periods. For Biomineralization assay human osteoblast cells were grown on a-C and SS samples for periods up to 14 days, deposition of mineralized matrix by osteoblasts cells was evaluated using scanning electron microscopy and scanning mapping x-ray analysis. X-ray microanalysis of the nodules demonstrated the bone-like nature of such a nodules, having Ca/P ratios around 1.6. to evaluate the ALP activity, HABDC cells were grown for 3, 7 and 14 days, the values obtained were higher in a-C films than steel uncoated. This study suggests that a-C films have a potential to use it in biomedical implants.

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Entidades citadas de la UNAM:

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Fuente:


ISBN:
1877040193
Editorial:

Tipo de producto:
Conference Paper