®®®® SIIA Público

Título del libro: Crispr And Rnai Systems: Nanobiotechnology Approaches To Plant Breeding And Protection: A Volume In Nanobiotechnology For Plant Protection
Título del capítulo: CRISPR-Cas epigenome editing: Improving crop resistance to pathogens

Autores UNAM:
JUAN CARLOS VIZUET DE RUEDA;
Autores externos:

Idioma:

Año de publicación:
2021
Palabras clave:

Cas12; CRISPR activation; CRISPR/Cas9; Defense genes; Epigenome editing; VIGE


Resumen:

CRISPR/Cas technology has been successfully designed for genome edition in numerous biological systems. Additionally, the re-engineering of Cas nucleases for purposes other than genome editing has resulted in the generation of, for example, catalytically inactive dead Cas9 or dCas12 nucleases, no longer able to cut any DNA strand, but with the ability to strongly bind the target sequence. Consequently, the different modifications to Cas9 and Cas12 nucleases offer a valuable platform to generate plant disease resistance against pathogens. In this chapter, we highlight some of the different modifications used to re-engineer Cas nucleases for targeted genome and epigenome editing, and their importance to modulate chromatin activity and gene expression. © 2021 Elsevier Inc. All rights reserved.


Entidades citadas de la UNAM: