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Título del libro: Capillary Electromigration Separation Methods
Título del capítulo: Electrophoretic methods for characterizing nanoparticles and evaluating their bio-interactions for their further use as diagnostic, imaging, or therapeutic tools

Autores UNAM:
GONZALO RAMIREZ GARCIA;
Autores externos:

Idioma:

Año de publicación:
2018
Palabras clave:

Biocompatibility; Biomedical applications; Colloidal stability; Drug delivery; Electrophoretic methods; Nano-bio interactions; Nanoparticles; Physicochemical characterization; Protein corona; Surface functionalization


Resumen:

Among the different methods commonly used to characterize nanoparticles (NPs), electrophoretic methods are an attractive and powerful tool that offers unique and precise physicochemical characterization of NPs for diagnosis, imaging, or therapeutic applications. Since electrophoretic methods, in capillary or microfluidic formats, provide high resolving power with short analysis times, require low sample consumption and produce reduced waste, and offer separations in free solution allowing to mimic in vivo conditions, they allow the characterization of nanomaterials in physiologically relevant buffers and the study of their biointeraction facilitating the prediction of their behavior for further biomedical applications. In this chapter, the fundamentals of electrokinetic methods are presented for these purposes. Some key applications are then presented to highlight the usefulness of this method for the design, synthesis, and surface functionalization control of nanomaterials, as well as for the study of their biocompatibility for biomedical applications. © 2018 Elsevier Inc. All rights reserved.


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