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Título del libro: Natural Oral Care In Dental Therapy
Título del capítulo: Cytotoxic and anti-inflammatory effect of turmeric and aloe vera in a gingivitis model

Autores UNAM:
RENE GARCIA CONTRERAS;
Autores externos:

Idioma:

Año de publicación:
2020
Palabras clave:

Aloe vera; Anti-inflammatory; Cytotoxicity; Gingivitis; Medicinal plants; Turmeric


Resumen:

The indiscriminate use of synthetic medicines has generated resistance to antimicrobials, and in response to this, special attention has been placed on medicinal plants. Objective: To know the cytotoxic and anti-inflammatory effects of turmeric and Aloe vera in human gingivitis-model. Materials and methods: Human gingival fibroblast (HGF) was subcultured in DMEM-supplemented medium. Turmeric and Aloe vera were inoculated (0-100 mg/mL and 0-25%). Viable cell number was determined by the MTT method and the mean cytotoxic concentration (CC50). Interleukin-1ß (IL-1ß) was used to induce a proinflammatory state. The anti-inflammatory effect was evaluated by the expression of prostaglandin E2 by an ELISA assay. Data were interpreted with ISO-10993-5 and analyzed with the normality tests of Shapiro-Wilk and Student t tests with a significance of 0.05. The data were made in triplicate from three independent experiments. Results: The cell viability of turmeric and Aloe vera showed a moderate cytotoxicity (p < 0.05) with CC50 values of 44.98 ± 1.4 mg/mL and 18.42 ± 2.1%, respectively. The expression of PGE2 significantly reduced the contact with turmeric and Aloe vera. Conclusions: Turmeric and Aloe vera have a moderate cytotoxic effect with an anti-inflammatory effect in HGF gingivitis model. The use of these medicinal plants has a promising potential clinical use for patients with gingivitis and periodontitis. © 2020 Scrivener Publishing LLC. All rights reserved.


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