®®®® SIIA Público

Título del libro: Epigenetics Methods
Título del capítulo: Monitoring genome-wide chromatin accessibility by formaldehyde-assisted isolation of regulatory elements sequencing (FAIRE-seq)

Autores UNAM:
JOSE ANTONIO NEME CASTILLO;
Autores externos:

Idioma:

Año de publicación:
2020
Palabras clave:

Bioinformatic analysis; Chromatin; Chromatin accessibility; Epigenomics; FAIRE-seq; Gene regulation; Genome-wide; Nucleosome; Transcription factor


Resumen:

Chromatin accessibility illustrates the density of nucleosome packaging and is a key feature of epigenetic analysis. Genomic DNA at accessible chromatin regions can be bound by transcription factors, polymerases and other nuclear proteins and often represents promoters, enhancers and other gene regulatory elements. In the past these sites have been identified via the hyper-sensitivity of chromatin for mild digestion with DNase I. Some 15 years ago the method formaldehyde-assisted isolation of regulatory elements sequencing (FAIRE-seq) has been developed, which allows the enzyme-free assessment of accessible genomic regions based on differential solubility in organic solvents. This chapter discusses the history, application and analysis of FAIRE-seq and provides examples from human immune cells. © 2020 Elsevier Inc. All rights reserved.


Entidades citadas de la UNAM: