®®®® SIIA Público

Título del libro: One Century Of The Discovery Of Arsenicosis In Latin America (1914-2014): As 2014 - Proceedings Of The 5th International Congress On Arsenic In The Environment
Título del capítulo: Characterization of Human hepatocellular carcinoma cells (HepG2), for experiments of effects the sex steroids hormones in arsenic metabolism

Autores UNAM:
ERICK SIERRA CAMPOS;
Autores externos:

Idioma:
Inglés
Año de publicación:
2014
Palabras clave:

Arsenic; Cell culture; Endocrinology; Metabolism; Antioxidant systems; Arsenic species; Cell lines; Cell viability; Hepatocellular carcinoma cell; Population studies; Sex steroid hormones; Sex steroids; Steroid hormones


Resumen:

Population studies showed that arsenic metabolism is more efficient in women, suggesting possible regulations by sex steroid hormones. This study evaluates the effect of testosterone, progesterone and estradiol on the profile of arsenic species in the cell line HepG2, which is able to metabolize 0.2 µM arsenite, to generate: 36.8% of iAs, 21.2% MMA and 42.0% DMA. This concentration of arsenic did not active the antioxidant system GSH/GSSG. Finally, the concentrations of steroid hormones were settled to no decrease cell viability below 80%. The effects of Testosterone 0.3-10 µg/dL, Progesterone 0.75-12 µg/dL and Estradiol 6.25-100.0 ng/dL were characterized. © 2014 Taylor & Francis Group.


Entidades citadas de la UNAM: