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Título del libro: Molecular Oncology Principles And Recent Advances
Título del capítulo: In vitro and in vivo models for cancer research

Autores UNAM:
JULIO ISAEL PEREZ CARREON;
Autores externos:

Idioma:
Inglés
Año de publicación:
2012
Palabras clave:

Cancer stem cells; Carcinogenesis; Cell culture; Chemical carcinogen; Cytochrome p450; DNA adducts; DNA damage; DNA transfection; Dysplasia; Gene silencing; Knockout mice; Metabolic activation; RNA interference; Solid tumors; Transgenic mice


Resumen:

Nowadays cancer kills thousands of humans around the world, so its research presents an important challenge to decrease cancer mortality. Strategies for cancer research have made great advancement in the last century, providing major insight into the complexity of tumor development. Numerous experimental protocols for many years have been performed to mimic features of cancer cells in humans; for example, to generate tumors in living organisms and study cancer in cultured cells. This chapter describes several of theses biological models: 1) chemical carcinogenesis protocols 2) genetically modified animals (transgenic and knockout mice), 3) cancer cell lines culture, 4) gene manipulation in cultured cells such as DNA transfection and RNA interference for gene knockdown and 5) the concept of cancer stem cells. The significance of in vivo and in vitro models for cancer research lies in the possibility of providing improved understanding of cancer biology and cancer treatment. © 2012 Bentham Science Publishers. All rights reserved.


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