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Título del libro: 2023 Ieee Embs R9 Conference, Embs R9 2023
Título del capítulo: Fabrication and Evaluation of Bioreactor to Decellularize and Recellularize Human Muscular Arteries

Autores UNAM:

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Autores externos:

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Idioma:

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Año de publicación:
2023

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Palabras clave:

3D printing; Additives; Bioconversion; Cell engineering; Radial flow; Sodium dodecyl sulfate; Sulfur compounds; Tissue; Tissue engineering; Additive print; Artery; Decellularization; Decellularized; Donor materials; Iliac arteries; Muscular arteries; Sodium dodecyl sulphate; Synthetic materials; Tissues engineerings; Bioreactors

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Resumen:

The typical vascular pathways utilized in many vascular procedures are either from donors or synthetic materials, frequently resulting in issues due to immune system suppression and limited longevity. Tissue engineering presents an innovative approach to produce personalized grafts featuring a natural extracellular matrix populated with recipient cells through the process of decellularization and recellularization. We show a detailed method to perform human iliac artery decellularization by radial flow perfusion; our radial flow bioreactor and their design and parametrization were published in other job. The artery was decellularized by with perfusion and recirculation of 1% of sodium dodecyl sulfate (SDS) for 7 days, with changes every 24 h. SDS was perfused at 35 mL/min at 25°C. The iliac artery was cleaned in ultrapure water with PBS. Washed again in PBS and preconditioned in endothelial medium and DNase. The decellularized artery with the bioreactor and the UNAM protocol appeared white in contrast to the normal vain. Hematoxylin and eosin (H&E) and DAPI staining demonstrated the presence of nuclei in normal and in decellularized arteries was better in the UNAM protocol than our bioreactor at same time; but the previous protocol uses 1750 ml of media against 490 ml. Our bioreactor, demonstrated to be an adequate and optimum decellularized system, we need just increase the perfusion time, in addition of providing efficient decellularized vain ready to Recellularize it in the same chamber. © 2023 IEEE.

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Entidades citadas de la UNAM:

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Fuente:


ISBN:
9798350381092
Editorial:
Institute of Electrical and Electronics Engineers Inc.

Tipo de producto:
Conference Paper

DOI:
10.1109/IEEECONF60929.2023.10525335