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Título del libro: The Ketogenic Diet Reexamined: Myth Vs. Reality
Título del capítulo: The regional expression of NKCC1 cotransporter in the dentate gyrus of rats fed with a ketogenic diet

Autores UNAM:
MIGUEL TAPIA RODRIGUEZ;
Autores externos:

Idioma:

Año de publicación:
2024
Palabras clave:

Dentate gyrus; Ketogenic diet; Laterality; NKCC1; Optical fractionator; Rat; Stereology


Resumen:

Ketogenic diet (KD) is a dietary regimen rich in fat, low in carbohydrates and adequate in protein. Initially, the diet was used as a nonpharmacological treatment in refractory epilepsy. However, in recent time, the diet has been shown to be effective in treating various neurological and psychiatric diseases associated with gamma aminobutyric acid (GABA) neurotransmitter dysfunction. NKCC1 is a cotransporter responsible for chloride inflow into the neurons. Thus, it participates in the regulation of intracellular chloride concentration that determines the effect of neurotransmission mediated by GABA. This study aimed to compare the regional distribution pattern of NKCC1 cotransporter expression in the dorsal/ventral and right/left dentate gyrus of rats fed with KD during a period from weaning to three months postweaning. At the beginning and the end of the treatment, the body weight and the blood levels of glucose and ß-hydroxybutyrate were measured. In addition, the expression of NKCC1 was analyzed by estimating the number of NKCC1 immunoreactive (NKCC1-IR) cells by stereological methods using the optical fractionator technique. At the end of treatment, the results indicated that the blood levels of ß-hydroxybutyrate were higher in KD-fed rats. However, the stereological count of NKCC1-IR cells in these rats did not show any difference in their regional distribution pattern in the dorsal/ventral and right/left dentate gyrus. These findings show that a three-month application of KD does not affect NKCC1 cation-chloride cotransporter expression in the dentate gyrus regions of the rats. © 2024 Nova Science Publishers, Inc.


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