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Título del libro: Gene-Edited Crops: The Crispr Solution For Global Food Security
Título del capítulo: CRISPR/Cas9: the Most Widely Adopted Tool for Genome Editing

Autores UNAM:
RUBEN DARIO ARROYO OLARTE; KARLA DANIELA RODRIGUEZ HERNANDEZ; RICARDO REYES CHILPA;
Autores externos:

Idioma:

Año de publicación:
2024
Palabras clave:

Bacteria; COVID-19; Genes; Genome; Phylogenetic; Proteomics; Tools; Archaeon; Bacterial genomes; Editing tools; Genetic materials; Nobel prizes; Non-native; RNA molecules; Specific segments; Two-component; DNA


Resumen:

CRISPR/Cas systems are the new generation of genome-editing tools, recognized with the Nobel Prize in Chemistry in 2020. CRISPRs are regions in the bacterial genome that help defend against plasmids and other non-native genetic materials (viruses). They are present in about 50% of bacteria and 90% of archaea and have two components: a protein called CAS for cutting DNA and a guide RNA molecule (gRNA) which together form a complex that recognizes and cuts specific segments of DNA. The editing process involves several stages to insertion or deletion of DNA, or to induce changes and replace a native gene with specificity and efficiency. This chapter discusses the use of CRISPR-Cas in the applications of genome editing. © 2025 selection and editorial matter, Aftab Ahmad, Nayla Munawar, and Baohong Zhang; individual chapters, the contributors.


Entidades citadas de la UNAM: